OptiPrep 250mL
Simplify the isolation and purification of macromolecules; viruses; a wide range of cell types; and organelles such as nuclei, mitochondria, endosomes, or exosomes by using OptiPrep™ medium. This flexible, versatile, and gentle density gradient medium is non-ionic, iodixanol-based (60% w/v) and has a density of 1.320 ± 0.001 g/mL. OptiPrep™ has been used clinically in X-ray contrast imaging and has been shown to have lower toxicity when compared to ionic gradient compounds such as metrizamide.
OptiPrep™ – 250 mL
High-Purity OptiPrep™ Density Gradient Medium (Iodixanol-Based)
Product Overview
OptiPrep™ (250 mL) is a ready-to-use, sterile, endotoxin-tested density gradient medium formulated with 60% (w/v) iodixanol in water, buffered with 10 mM Tricine and 0.85% NaCl at pH 7.2. Developed and manufactured by Axis-Shield, this solution is trusted globally for its performance in isolating cells, subcellular organelles, viruses, and proteins using isosmotic gradients.
Whether you’re performing cell purification, exosome isolation, or virus concentration, OptiPrep™ density gradient medium ensures exceptional resolution, reproducibility, and biocompatibility—making it an essential reagent in molecular biology, immunology, and virology labs.
Key Features of OptiPrep™:
✅ Pre-made 60% iodixanol solution
✅ Compatible with a wide range of biological samples
✅ High-purity and low-endotoxin certified
✅ Non-toxic and iso-osmotic to cells
✅ Easy to dilute to required OptiPrep™ gradient concentrations
Applications
- Exosome and microvesicle purification
- Isolation of mononuclear cells and PBMCs
- Vir us purification (adenovirus, lentivirus, etc.)
- Mitochondria, nuclei, and membrane vesicle separation
- Protein and lipoprotein fractionation
This product is ideal for researchers using ultracentrifugation or gentle gradient separation protocols where precise control over buoyant density is critical.
What is OptiPrep™?
OptiPrep™ is an iodixanol-based density gradient medium with superior clarity, ideal osmolality, and excellent biocompatibility. Unlike other separation media, OptiPrep™ iodixanol remains non-ionic and inert, ensuring that your target biological material remains intact and functionally active throughout the isolation process.
Looking to prepare your own OptiPrep gradient? Simply dilute with a suitable buffer to achieve the desired density. OptiPrep™ gradients are highly stable and allow efficient separation of cells and particles based on subtle density differences.
Technical Specifications
- Volume: 250 mL
- Concentration: 60% iodixanol (w/v)
- Density: 1.320 g/mL
- pH: 7.2
- Buffer: 10 mM Tricine, 0.85% NaCl
- Sterility: Sterile filtered
- Storage: 2–8 °C (do not freeze)
- Manufacturer: Axis-Shield
Why Buy OptiPrep™ from Axis-Shield?
Axis-Shield is the original developer and global supplier of OptiPrep™ density gradient solutions, offering unmatched reliability and performance. All lots are quality-controlled for density, pH, osmolality, and endotoxin levels, providing peace of mind for sensitive experiments.
Explore More
Searching for high-quality OptiPrep iodixanol, pre-made OptiPrep gradient, or other gradient media?
Visit our full catalog of OptiPrep™ Density Gradient Media to find the right formulation for your needs.
Order Now to get fast delivery and guaranteed performance with Axis-Shield’s OptiPrep™ – the gold standard in density gradient separation.
Simplify the isolation and purification of macromolecules; viruses; a wide range of cell types; and organelles such as nuclei, mitochondria, endosomes, or exosomes by using OptiPrep™ medium. This flexible, versatile, and gentle density gradient medium is non-ionic, iodixanol-based (60% w/v) and has a density of 1.320 ± 0.001 g/mL. OptiPrep™ has been used clinically in X-ray contrast imaging and has been shown to have lower toxicity when compared to ionic gradient compounds such as metrizamide.
CONTAINS
• Iodixanol: 60% (w/v)
• Density: 1.320 ± 0.001 g/mL
CELL TYPE
Mononuclear Cells, Other
SPECIES
Human, Mouse, Rat, Non-Human Primate, Other
APPLICATION
Cell Isolation
BRAND
OptiPrep
AREA OF INTEREST
Immunology205
https://maxanim.com/density-gradient-media/optiprep/
Optiprep is a type of density gradient medium that is used in laboratory settings to separate cells or other biological particles based on their size and density. It is commonly used in a technique called density gradient centrifugation, which involves spinning a tube of Optiprep and other components at high speeds to create a gradient of increasing density. This gradient allows cells or other particles to be separated based on their density and size, with heavier or denser particles settling towards the bottom of the tube and lighter or less dense particles remaining towards the top.
Optiprep is a specific brand of density gradient medium that is made by Sigma-Aldrich, a company that specializes in supplying chemical, biological, and pharmaceutical products to researchers and other professionals. It is commonly used in a variety of applications, including blood transfusion, tissue culture, and gene expression analysis. Optiprep is also used in research and diagnostic laboratories to purify or isolate cells or other biological materials, such as viruses or proteins.
lymphoprep
Lymphoprep is a type of density gradient medium that is used in laboratory settings to separate cells.
OptiPrep™ is a sterile and endotoxin
tested solution of 60%
iodixanol in water with a density of 1.32 g/ml.
Iodixanol was developed as an X-ray contrast medium an has therefore been subjected to rigorous clinical testing.
Iodixanol is non-ionic, non-toxic to cells and metabolically inert.
Iodixanol solutions can be made isoosmotic at all useful densities.
Iodixanol solutions have low viscosity and osmolality.
Actual endotoxin levels in each batch are usually measured at < 0.13 EU/ml.
The high density of OptiPrep™ facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient or through a simple density barrier. 55 protocols available for cell isolation.
Improved resolution of cell organelles.
Low viscosity, isoosmotic gradients
provide rapid and efficient separation
of the major organelles in preformed
gradients.
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